7.002 | Spring 2025 | Undergraduate

Fundamentals of Experimental Molecular Biology

Lab Objective 4: Analysis of ClpX Protein Purification

Lab Day 8

  • 4.1: Run samples on two SDS-polyacrylamide gels
  • 4.2: Western transfer one SDS-polyacrylamide gel
  • 4.3: Coomassie-stain second SDS-polyacrylamide gel

Lab Day 9

  • 4.4: Rehydrate western blot, block and probe with primary antibody
  • 4.5: Destain Coomassie-stained SDS-polyacrylamide gel
  • 4.6: Wash and detect western blot

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Spring 2025
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