Lab Day 6
- 3.1: Analysis of sequencing data
- 3.2: Transformation of E. coli LOBSTR BL21(DE3) with mutant clpX plasmids
- 3.3: Make purification buffers.
Lab Day 6.5
- 3.4: Expression of ClpX mutant protein and collection of cell pellet
*** Note: Part 6.5 will be performed by the 7.002 teaching staff
Lab Day 7
- 3.5: Analysis of E. coli LOBSTR BL21(DE3) transformations
- 3.6: Purification of mutant ClpX ATPase protein
- 3.7: Determination of purified mutant ClpX protein concentration
