Lab Day 2
- 2.1: Preparation of “Round-the-horn” PCR mutagenesis reactions
Lab Day 3
- 2.2: Gel analysis of PCR mutagenesis products
- 2.3: Purification of PCR mutagenesis products
- 2.4: Digestion, phosphorylation, and ligation of purified PCR products
Lab Day 4
- 2.5: Transformation of E. coli DH5α with PCR ligations
Lab Day 5
- 2.6: Miniprep of putative mutant clpX plasmid DNA from bacteria
- 2.7: Preparation of clpX miniprep DNA for sequencing
- 2.8: Analysis of E. coli DH5α PCR transformations
