The manual contains the experiments and background information for the course and details the procedures to be completed during each day in the lab. The experiments in this course are divided into 10 days spent in the lab, followed by extra material in the appendix.
| Lab Manual Sections | Pages |
|---|---|
| Day 1 | 5–16 |
| Day 2 | 17–20 ; 21–27 |
| Day 3 | 28–30 ; 31–38 |
| Day 4 | 39–43 |
| Day 5 | 44–51 |
| Day 6 | 52–60 |
| Day 6.5 | 61–62 |
| Day 7 | 63–75 |
| Day 8 | 76–85 |
| Day 9 | 86–92 |
| Day 10 | 93–102 |
| APPENDIX | |
| Lab Safety Guidelines | 104–107 |
| Eppendorf BioSpectrometer Instructions | 108–109 |
| Molecular Weight Markers and Amino Acid Structures | 110 |
| Codon Chart | 111 |
| Primers Available for RTH Mutagenesis | 112 |
| ClpXdeltaN Coding Sequence | 113–114 |
| pET Vector Cloning Site Sequence | 115 |
| Plasmid Maps and Strain Info | 116 |
Note: In the on-campus course, students completed their pre-labs on an internal 7.002 MITx site, which is not represented here. They interactively learned the background of lab techniques and answered some questions to ensure they understood the lab manual and experimental setup before coming to lab.
